p akt dyc887b 2 kits  (R&D Systems)

Journal: Communications Chemistry

Article Title: Sustained CREB phosphorylation by lipid-peptide liquid crystalline nanoassemblies

doi: 10.1038/s42004-023-01043-9

Figure Lengend Snippet: a LNP cellular uptake measured by flow cytometry: Internalization of Rhodamine R18-labeled MO cubosomes (10 µM) in differentiated SH-SY5Y cells after 24 h of incubation (determined by FL2 fluorescence intensity). b Cellular uptake of PACAP: Internalization of PACAP-TAMRA (5 µM) in differentiated SH-SY5Y cells at different incubation times of 3 h, 6 h and 24 h (determined by FL2 fluorescence intensity). c Cellular uptake of LNP-PACAP: Internalization of LNP-PACAP-TAMRA by differentiated SH-SY5Y cells from 5 µM PACAP-TAMRA/10 µM LNPs mixture at different time points 3 h, 6 h and 24 h. The data of ( a – c ) was presented as a ratio of the mean intensity of FL2 in the treated group ( n = 3) and the mean intensity of FL2 in the RA/FBS(-) control group. d Kinetic changes of CREB phosphorylation in the PD model. The ELISA assay was performed with plasmalogen-loaded PL-C LNPs, which were dispersed at total lipid concentration of 10 µM in the presence of 5 µM PACAP peptide. The different incubation times were 0, 3 h, 6 h, and 24 h following 30 min 6-OHDA exposure. e Kinetics changes of phospho-AKT expression levels in the treated PD model. ELISA assay was performed with plasmalogen-loaded nanoparticles PL-C, which were dispersed at same total lipid concentration of 10 µM in the presence of 5 µM PACAP. f Kinetic changes of phospho-ERK expression in the treated PD model measured by ELISA assay with plasmalogen-loaded nanoparticles PL-C at total lipid concentration of 10 µM in the presence of 5 µM PACAP peptide. The data of ( d – f ) is presented as a ratio of the quantity of p-CREB, p-AKT or p-ERK expression (ng) per total protein (ng) in the treated group ( n = 3) and the quantity of p-CREB expression (ng) per total protein (ng) in the 6-OHDA group ( n = 3). n indicates the number of replicates per group and the error bars represent the standard deviation. * P ≤ 0.05, ** P ≤ 0.01, and *** P ≤ 0.001.

Article Snippet: DYC1018B-5, Bio-Techne Ltd./R&D Systems, UK), and Phospho-AKT (S473) Pan Specific DuoSet IC ELISA kits (Cat. #.

Techniques: Flow Cytometry, Labeling, Incubation, Fluorescence, Enzyme-linked Immunosorbent Assay, Concentration Assay, Expressing, Standard Deviation

Journal: Bioscience Reports

Article Title: Paracrine interleukin-8 affects mesenchymal stem cells through the Akt pathway and enhances human umbilical vein endothelial cell proliferation and migration

doi: 10.1042/BSR20210198

Figure Lengend Snippet: ( A ) The content of VEGF protein in the supernatant of BMSCs was determined by ELISA. * P <0.01, # P <0.05, △ P <0.01 ( n =15). ( B ) The content of IL-6 protein in each BMSC supernatant was determined by ELISA. * P <0.01, # P <0.01, △ P <0.01 ( n =15). ( C ) The content of Akt protein in BMSC lysates was determined by ELISA. * P <0.01, # P <0.01, △ P <0.01 ( n =15). ( D ) The content of P-Akt protein in each BMSC cohort was determined by ELISA. * P <0.01, # P <0.01, △ P <0.01 ( n =15).

Article Snippet: The BMSC lysates’ contents of Akt and phospho-Akt (P-Akt) in each group were determined by human-Akt (cat. no. DYC1775-5), P-Akt (cat. no. DYC887B-5), and ELISA kit (All R&D, MN, USA).

Techniques: Enzyme-linked Immunosorbent Assay